Microbiology Unknown and Purpose of Microbiology Essay - Paper Example Microbiology Unknown and Purpose of Microbiology Essay - Part 3 Microbiology Unknown 94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class - Microbiology Unknown and Purpose of Microbiology Essay introduction. It is possible to identify a mixed culture by running experiments on the unknown bacteria and taking what we already know and applying it to the results. It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests.
Contact Author Why identify a bacteria? Bacteria are everywhere, they are part of our environment and even of us. In fact, we are more bacteria than human! Indeed, we have approximatively human cells and bacterials cells in us.
Therefore, we encounter bacteria everywhere and it is sometimes necessary to identify them. Whether it is to determine the cause of a disease, to test if a certain food is safe to eat or simply to know what is present in a certain ecosystem, we have developed many techniques to identify bacteria.
Bacteria may seem like very simple organisms and you might think that most of them share many characteristics. In fact, every species is unique and has particular characteristics. This makes it possible to identify an unknown species.
In this article, I'm going to go over some of the simple tests you would perform on your unknown in order to identify it. Source First some basics Before going over the tests to identify an unknown bacterial species, we should remember some bases of manipulating bacteria.
It is important to always keep in mind that your unknown species is a potential pathogen. This means that it could be harmful to you. Therefore, when working with bacteria you must wear a lab coat, safety glasses and gloves. If you suspect that your bacteria might be an airborne pathogen depending on where it comes from: Moreover, you must use the proper aseptic techniques to keep out all unwanted organisms from your culture.
If you use a loop or a needle to transfer bacteria from a medium to another, you must flame the loop or needle in the flame of a Bunsen burner for a few seconds and then wait for the wire to cool down to avoid killing your bacteria.
You must always work in the area around our flame since microorganisms are present in the air. The area around the burner can be considered sterile.
If you transfer your bacterium to or from a tube, you should flame the neck of the tube for a few seconds before and after. It creates a convection current and kills the cells that might have fallen in it during the manipulation.
Bacteria are cultured in either liquid or solid medium. Both contain agar, which is composed of complex polysaccharides, NaCl and yeast extract or peptone.
Now that the basics are covered we can move on to start testing on our bacteria to determine which species it might belong to! Example of a particular culture morphology Source Culture morphology When find an unknown bacterium, you first make a pure culture of it on an agar plate.
A pure culture arrises from a single cell and thus contains only one type of microorganism. A colony is a visible mass of cells.
Different bacterial species creates different culture morphologies. You can focus on the form, elevation, margin, surface, optical characteristics and pigmentation of your culture to describe it. Some species make very particular colonies.
For example, Serratia marcescens forms bright red colonies and can be easily identified thanks to this pigmentation. Unfortunately, a lot of bacteria have very common colonies round, flat and white or creamy white and this test is not enough to identify with certainty a species.
But it's still a very useful first step and helps progress in the identification of the bacteria. It's mostly a technique to rule out some options and to make sure we are dealing with a bacteria and not for example a mold. Cell morphology The second step to your identification is to put your unknown on a microscope slide and observe the morphology of your cell.
The most common shapes are: Coccus round Vibrio comma-shaped Spirochete spirale But some bacteria have very unique shapes and are therefore highly identifiable like that.The first day an unknown sample was assigned to each group of students.
The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded.
Then the sample was put on agar plates using. The importance of identifying these pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period.
The Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many.
Identifying Two Unknown Species of Bacteria Essay - Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November ) The first day an unknown sample was assigned to each group of students.
The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The differential tests used to identify the unknown cultures were Gram staining, oxidase, indole test, urea test, and casein test.
MATERIALS AND METHODS: The unknown bacteria were given out by the lab instructor. Each student chose their own unknown bacteria according to the number. There are two main types of bacteria, gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number The following are the tests that were used to identify the two different bacteria.
Identification of Two Unknown Bacteria Abstract The purpose of this experiment was to learn how to isolate and identify unknown bacteria using methods from the lab.
Unknown 9 was used for this experiment.